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Defibrinated sheep blood sigma

Description. Be confident that your animal blood meets the highest standards possible by using Thermo Scientific™ Defibrinated Sheep Blood, sourced from our own technologically-advanced sheep farm. Videos. Chemical structures Bovine / Horse / Porcine / Rabbit / Sheep Our defibrinated blood products are aseptically drawn from healthy, donor animals of U.S. origin. Blood is mechanically defibrinated without anticoagulants or other additives. All material is screened prior to being pooled for color, PCV and hemolysis Red blood cells from sheep dry powder (glutaraldehyde treated); find Sigma-Aldrich-R3378 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldric

Defibrination of sheep blood, aseptically collected from the jugular vein of a healthy sheep, can be obtained, processing a mechanic agitation (manually) using sterile glass pearls (2 pearls of 4.. Defibrinated sheep blood is ideal for blood agar plate preparations. Blood Agar is a general purpose enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties Aldrich, >90 %), defibrinated sheep blood (Thermo Scientific Oxoid, Fisher Scientific), Triton X-100 (Sigma-Aldrich), calf bovine serum (CBS, Sigma-Aldrich, Sterile-Filtered), foetal bovine serum (FBS, Sigma-Aldrich) were used as received. Dulbecco's Modified Eagle Medium (DMEM), phosphate-buffered saline (PBS), L- glutamine and sterile water. 10145-962EA 89.51 USD. 10145-962. Blood, Sheep, Defibrinated, Hardy Diagnostics. Blood. The sheep are humanely bled and rotated on a veterinary supervised program. Derived form a donor herd. Antibiotic-free. Less prone to spontaneous hemolysis. This product has a short shelf life and ships from our California warehouse only

The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin Defibrinated sheep blood is ideal for blood agar plate preparations. Blood Agar is a general purpose enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. Related Products: Sheep Blood Horse Blood, Defibrinated, 30 mL, screw cap bottle 30 mL / Bottle: 4290-1001-10008 Sheep Blood, Defibrinated, 100 mL serum bottle 100 mL / Bottle: 4290-2040-50024 Bovine Serum, sterile filtered, 500 mL, PETG bottle 500 mL / Bottl

Defibrinated Sheep Blood - Thermo Fisher Scientifi

Defibrinated Blood Bovine Horse Rabbit Sheep Hemostat

Mosquitoes were blood-fed using an artificial blood feeder (CG-1836, Chemglass Life Sciences, USA) filled with defibrinated sheep blood (#337, HåtunaLab, Sweden) (heated to 37°C), spiked with 10mM ATP (A1852, Sigma-Aldrich) for about 2 h per cage. Blood-fed mosquitoes were subsequently allowed to feed on 10% sucrose solution Sterile defibrinated sheep blood used to enrich the medium produces the hemolysis characteristics of different bacteria, such as streptococci, listeria, hemolytic Staphylococcus, Escherichia coli and Pseudomonas. Not all products are available for sale in all territories. Please inquire

Red blood cells from sheep - Sigma-Aldric

  1. Research. Development. Production. We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production
  2. Rockland Immunochemicals SHEEP BLOOD Defibrinated - R111-0100. Defibrinated sheep blood is aseptically collected and prepared to order from our colony of animals. Fibrin is mechanically removed during the clotting process without the presence of anticoagulants or other additives. Defibrinated sheep blood is ideal for blood agar plate preparations
  3. Adult female C. sonorensis (n = 200) midges were allowed to feed on defibrinated sheep blood mixed 1:1 (v/v) with SARS-CoV-2 (2.0 × 10 6 TCID 50 /ml) using a bell jar method with parafilm membrane. Negative control unfed midges (n = 100) were maintained in adjacent cages
  4. 1 800 255 6730 www thermoflsher com. 1. About Us. Thermo Fisher Scientific Inc is the world leader in serving science Our mission is to enable our customers to make the worl
  5. blood (sheep, horse) to prepare blood agar for the isolation of fastidious organisms like Brucella. Dextrose is the source of energy. Tryptose serves as nitrogen source while sodium chloride maintains osmotic equilibrium. Blood Agar may be prepared by adding 5%v/v sterile defibrinated blood t
  6. Thermo Scientific Oxoid 100 ML HORSE BLOOD DEFIBRINATED 100ML Brand: Thermo Scientific Oxoid SR0050C 100 ML HORSE BLOOD DEFIBRINATED 100ML. Code : 50. Additional Details : Weight : 0.15000kg.
  7. The assay was performed on Mueller Hinton Agar plates supplemented with 5% defibrinated sheep blood (Thermo Scientific, Milan, Italy). Ampicillin (10 μg) and tetracycline (30 μg), were applied as antibiotic agents following Clinical and Laboratory Standards Institute [CLSI] recommendations for fastidious organisms (document M45-A2)

Where can I get defibrinated sheep blood

For the whole blood measurements, defibrinated sheep blood (Remel animal blood purchased in Sigma-Aldrich) was used. The Remel animal blood meets all standards for laboratory tests. The glucose concentration in the defibrinated blood was determined using tests strips (Accu-Chek, Roche Diagnostics), as the average of three consecutive. inoculum. Defibrinated sheep blood agar was used as the medium. The minimum inhibitory concentrations (MICs) of 5 antimicrobial agents namely, penicillin G (Sigma), clindamycin (The Upjohn Co. Mich), vancomycin (Eli Lilly & Co, Indianapolis), metronidazole (Sigma) and ampicillin-sulbactam (Pfizer, Conneticut) were determined Other products used in the isolation of Clostridium difficile : . CM0601 Clostridium difficile Agar Base; SR0050 Horse Blood, Defibrinated; SR0051 Sheep Blood, Defibrinated; SR009 (TSB) containing 5% defibrinated sheep blood at 37 C ° with 5% CO 2 for 18 h and maintained in a deep freezer (-70 °C) in the same medium containing 20% (v/v) glycerol. 2.2. Culture Medium and bacterial growth Conditions The Hoeprich's modified medium (HMM) was used for the cultivation. Each liter of this medium contains 20 Thereafter, cell culture media was collected and mixed with an equal amount of defibrinated sheep blood, 40% BSA (Sigma Aldrich, St. Louis, MO) or 2x PBS (pH 7.4, Sigma) including 10 mM ATP. One week-old females that had been deprived of sugar for 24 h were fed for 1 h with a mixture consisting of CHIKV-infected cell culture and blood, BSA, or.

supplemented with 5% defibrinated sheep blood® (E&O laboratories, Bonnybridge, Scotland), 100 mg/L Neomycin®, 5 mg/L Vancomycin® and 1 mg/L Erythromycin® (Sigma-Aldrich, Saint Louis, Missouri), and incubated anaerobically for 3 days at 37°C. Based on previous descriptions of the colony morphology o The cells were revived on defibrinated sheep blood, dehydrated Schaedler anaerobe agar plates. After inoculation from the initial plate aseptically (without any oxygen prevention), the inoculated plates were placed in a 2.5 L anaerobic jar using Anaerocult A to generate an anaerobic environment and incubated at 37℃ taurocholate hydrate (Sigma) and 5% defibrinated sheep blood (Oxoid). Plates were incubated anaerobically at 37°C for 48h in an Oxoid 2.5l AnaeroJar plus Anaero-Gen sachet. Resulting colonies were examined and for each morphologically distinct isolate, four colonies were subcultured by streaking onto Colombia agar with sheep Fetal Calf Serum. Donor Horse (Equine) Serum. Human Serum and Plasma. Selectrol ®. Accu-Bac Quantitative Micro-organisms. Human Platelet Lysate (HPL) Cardinal Biologicals Inc. We carefully select strategic partners from around the globe in order to ensure that we can provide serum products of the highest quality for any application

EO Labs Defibrinated Horse Blood (Universal Polystyrene Bottle) - 25mL. Code: MED1300 D2-65. Freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture me... read more. ADD. £5.54 EACH. List Price 414 nm for detection of haemolysis in red blood cells.12 The cells we used for this technique are red blood cells from defibrinated sheep blood since the heme group of hemoglobin Fig. 1 (a) The nanostructured mechanical filter is shown in a photograph. (b) A schematic is shown of the mechanical lysis portion of the device tha Blood agar is an excellent medium for the cultivation of fastidious bacteria that require particular nutrients and don't profusely grow on general media like Nutrient Agar. About 5% of defibrinated mammalian blood (human, sheep, or horse) is added to the autoclaved basal media to prepare blood agar medium A leading provider of top quality blood and tissue products for research, BioChemed offers a range of custom and standard services for serum and plasma. (540) 667-3348 Request a Quote Products & Service Defibrinated Sheep Blood. 10 L - Find MSDS or SDS, a COA, data sheets and more information

Sheep Blood Defibrinated R111-0050 - rockland-inc

  1. supplemented with 5% defibrinated sheep blood and 5µg/ml of gentamycin (Sigma Chemical Co., St. Louis) and incubated in 5 % CO 2 at 36 ºC by 24 h. The maintenance of the bacterial strain was accomplished in Brain Heart Infusion (BHI) broth (Oxoid, Basingstoke, UK) with 20 % glycerol at 4 ºC (18). Th
  2. Selected Lactobacillus strains were analysed for their haemolytic activity on MRS agar with 5% (v/v) defibrinated sheep blood (Graso® Biotech, Starogard Gdański, Poland), based on the method previously described by Guerra et al. . Each strain was analysed in two modes of inoculation: streaking and stabbing into the agar, in three repetitions
  3. imizes misidentification of colonies, thereby reducing specimen processing cost and turnaround time, and helps accuracy of reports. 4 Defibrinated sheep blood is largely.

For example, blood agar plates (BAP) are made by enriching TSA plates with defibrinated sheep blood, and chocolate agar is made through additional cooking of BAP. Nutrient agar is also similar to TSA. One liter of the agar contains: ^ Sigma aldrich nonselective media A sterile cotton swab was dipped in the bacterial suspension and swabbed over the surface of the Mueller-Hinton agar plates supplemented with 5% defibrinated sheep blood (MHAB) (bioMérieux SA, Marcy-l'Etoile, France). The antibiotic disks were placed on the plates and incubated for 20-24 h at 35 °C in a 5% CO 2 atmosphere 5% defibrinated sheep blood and incubating under the conditions named before. The antibacterial activity was evaluated by measuring in milli-meters the diameter of the inhibitory zone. All experiments were conducted in triplicate and the mean± SD was calculated for each strain. The standard reference penicillin (Sigma Aldrich Co.; Madrid Serotype 3 ATCC 6303 strain bacteria were grown in Trypticase soy agar with 5% defibrinated sheep blood or brain heart infusion (BD, Franklin Lakes, NJ, USA) in a 5% CO 2 atmosphere. Subsequently, the bacteria were pelleted, heat killed in phosphate buffered saline (PBS) at 56°C for 45 min and further digested in a 0.2% pepsin solution for 2 h. 5% defibrinated sheep blood. 50.0. ml. Distilled water. 1.0. L. After preparing the Trypticase soy agar plate with 5%sheep blood, spreading the subculture on the agar surface and place one NAM disk aseptically. Incubate the agar plate under a H 2 -CO 2 -N 2 (1:1:8) gas mixture. To prepare the NAM disk, impregnate with 20 µl filter-sterilized 1.

Blood, Sheep, Defibrinated, Hardy Diagnostics VW

DHB - Defibrinated Horse Blood - E & O Laboratories Lt

Defibrinated Blood at Thomas Scientifi

Four-day old adult female Aedes simpsoni sl. were deprived of water and glucose for 12 hours before they were orally exposed to infectious blood meal mixed with either quantified BUNV (10 5.18 PFU/ml) or NGIV (10 7.42 PFU/ml) and defibrinated sheep blood at a ratio of 1:1. The exposure time was one hour, using parafilm as an artificial membrane. from Sigma-Aldrich. Amino-PEG5000 thiols, azide-PEG5000 thiols and heterobifunctional linker succinimidyl- ([N-maleimidopropionamido]-diethyleneglycol) ester (SM(EG)2) were purchased from Ponsure Biotech, Inc (Shanghai, China). Sterile defibrinated sheep blood an were directly streaked onto blood agar base (Himedia, Mumbai, Maharashtra, India) supplemented with 5% defibrinated sheep blood. After aerobic incubation at 37°C for 24-48 h suspected colonies were selected for further identification [14]. Pure cultures were obtained from these isolates after studying morphologica Samples of 10 −6 and 10 −7 dilutions were plated on agar medium supplemented with 5% defibrinated sheep blood (Columbia Blood Agar, Poland). The medium was prepared by dissolving 43 g of dry base mixture (Columbia Agar Base, Grasso Biotech, Poland) containing casein hydrolyzate (5.0g), meat extract (8.0g), yeast extract (10.0g), sodium.

Animal Blood Products Innovatek Medical Inc

  1. (Sigma-Aldrich, Saint Quentin Fallavier, France) and 1% (v/v) of vita
  2. , the diameter of the clearing zones around the colonies was measured. Hemolytic activity assay was performed by adding 5% defibrinated sheep blood (Oxoid, Basingstoke, Hampshire, UK) into autoclaved LB 35 agar.
  3. (5mg/ml) and vita
  4. , and L-cystine. The invention features an all-purpose microbiological growth media that can support the growth of anaerobes, molds.
  5. Fresh samples were immediately transferred to an anaerobic chamber (Bactron I-II Shellab, USA) with an atmosphere of 85% N 2, 5% CO 2, and 10% H 2 at 37°C and streaked on Columbia agar plates (Oxoid, UK) supplemented with 5% defibrinated sheep blood (Novamed, Israel) and 0.15% crystal violet. Large resection samples were dissected with a.
  6. Blood Agar (BAP): consists of a basal medium such as TSA enriched with 5% defibrinated sheep blood or in some locations, horse blood. What is red blood cell hemolysis? Hemolysis , also spelled haemolysis , also called hematolysis, breakdown or destruction of red blood cells so that the contained oxygen-carrying pigment hemoglobin is freed into.

Sheep Blood Agar Millipore Bio

  1. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended
  2. A composition for use in inhibiting or killing Porphyromonas gingivalis, comprising an effective amount of Antrodia camphorata powder or extract and acceptable carriers thereof for inhibiting or killing Porphyromonas gingivalis. Also provided is a method of inhibiting or killing Porphyromonas gingivalis by using aforementioned composition
  3. 9. Sheep blood, defibrinated 10. Ethanol, absolute 11. Fluorescent antibody (FA) buffer (Difco) 12. Glycine anhydride 13. Gram stain kit 14. Hydrogen peroxide solution, 3% for catalase test (R12) 15. KOH 40% solution (R65) 16. Listeria-typing sera set (Difco) 17. Lithium chloride-phenylethanol-moxalactam (LPM) agar (M81) with added esculin and.
  4. (Sigma) and menadione (Biochemical); and Brain heart infusion broth (Difco) supplemented with he
  5. ants The Indian Journal of Small Ru
  6. (Sigma). 200 µg/ml FUdR (Sigma), 25 µg/ml erythromycin (Sigma), and 200 µg/ml gentamycin (Sigma) where added before pouring the plates when needed. 7 . Bacterial culture and mono-colonized mic

Physical and composition characteristics of clinical

5% defibrinated sheep blood, 0.0005% hemin (Sigma-Aldrich Gallarate), and 0.00005% mena-dione (Sigma-Aldrich Gallarate). Four wells, each 0.65 mm in diameter, were made in each agar plate, and the pits were filled with 70 µL of the extracting solution from all specimens obtained at the differ-ent times. PBS was used as the negative control and. 116 provided with 10% sugar solution ad libitum and an opportunity to feed on defibrinated sheep 117 blood once per week. An Ae. notoscriptus colony was established from eggs collected in Brisbane, (Sigma-Aldrich) in formaldehyde (1% v/v) and methanol (1% v/v) Adult female C. sonorensis (n=200) midges were allowed to feed on defibrinated sheep blood mixed 1:1 (v/v) with SARS-CoV-2 (2.0 x 106 TCID 50/ml). Negative control unfed midges (n=100) were maintained in adjacent cages. For mosquitoes, 8-day old Cx. tarsalis (n=100) o agar (Oxoid) supplemented with 5% (v/v) defibrinated sheep blood and colistin (10 mg/L) and incubated anaerobically for 3 d. Bacteria from the final selective agar were then identi-fied by MALDI-TOF MS. Bacterial isolate identification by MALDI-TOF analysis Proteins from bacterial colonies from the final selective aga Then, 0.1 mL of the enrichment selective broth was plated onto Wilkins Chalgren anaerobe agar (Oxoid, UK) supplemented with 5% of defibrinated horse blood and 30 mg/L colistin methanesulfonate, 100 mg/L cycloheximide, 30 mg/L nalidixic acid, 30 mg/L trimethoprim, and 10 mg/L vancomycin (Sigma, USA) and incubated for seven days at 37°C under.

Comparison of bactericidal and fungicidal efficacy of

The irritant receptor TRPA1 mediates the mosquito

ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood. Temperature. 37°C. Handling procedure. 1. Open vial according to enclosed instructions. 2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet. (SIGMA-70), partial cds. For this purpose, samples were plated on blood agar (Oxoid®) supplemented with 5% defibrinated sheep blood and incubated at 37 °C for 24 h under microaerobic conditions. The initial identification was done based on phenotypic and biochemical properties and was further confirmed by detection of the kmt1 gene in a species-specific PCR assay [ 35 ] Sterile defibrinated sheep red blood cells 9 (RBCs) were provided by Guangzhou Future Biotechnology, China. Anhydrous trehalose 10 (Tre) and trifluoroacetic acid (TFA) were obtained from J&K Scientific Ltd., China. Sodium 11 hydroxide, 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), anhydrou sion agar, blood agar base (No. 2) and egg yolk emul-sion agar supplemented with 5% defibrinated sheep blood, campylobacter selective supplement, skirrow powder (Cat. No. CSS80004, Biolab, Hungary), and amphotericin B solution (250 μg/mL, 1 ml/L) (Cat. No. A2942, Sigma) were added aseptically to 500 m

Blood Agar (TSA with Sheep Blood) Mediu

Campy blood agar (MCBA) plates containing Brucella agar base (Merck, Germany), supplemented with 7% defibrinated sheep blood and antibiotics (polymyxin B, amphotericin B, vancomycin) for 3-7 d. The resulting colonies were identified by Gram staining, biochemical tests and polymerase chain reaction (PCR) usin Briefly, prior to oral DENV infections, 6- to 7-day-old adult female mosquitoes were sorted and starved for 24 h. For mono-infections, a 1:1 mix of virus culture and defibrinated sheep blood was prepared. For co-infections, 1 ml of each DENV serotype was combined, and from that blend, 1 ml was combined with 1 ml of defibrinated sheep blood and 0.1% sodium taurocholate; Blood, 5% defibrinated sheep blood. †Poor test agreement was found among and between cultures ( -0.28; p>0.9). ‡Culture sensitivity calculation based on parallel interpretation of all 4 cultures (standard comparator) and 6.1% of overall contamination The optimal decontamination method for needle-free connectors is still unresolved. The objective of this study was to determine if a continuous passive disinfection cap is as effective as standard cleaning for the microbial decontamination of injection ports of two types of needle-free connectors. The injection ports of needle-free connectors were inoculated with Staphylococcus aureus and.

1-octanol Sigma-Aldric

  1. Serial dilutions of fermented feces were plated in fastidious anaerobe agar media plates containing 0.5% defibrinated sheep blood and filtered (0.22 µm) and fermented intestinal bacteria medium, used as a nutritional supplement (0.1 mL of medium per agar plate). Dilutions were then incubated at 37°C for 72 hours in an anaerobic chamber
  2. Columbia Agar with 5% Sheep Blood or BD Trypticase Soy Agar II with 5% Sheep Blood and, eventually, on additional selective and nonselective media. Usually, an incubation temperature of 35 ± 2° C is adequate. Incubate for 18 to 24 h or longer if required. For use as a suspension medium, inoculate the tube with a small amount of growt
  3. Brand: Thermo ScientificTM OxoidTM Cultivate and isolate fastidious pathogens and other microorganisms with Thermo Scientific™ Oxoid™ Blood Agar Base No. 2 (Dehydrated). The specially designed formulation meets the need for a nutritious blood agar which permits maximum recovery of delicate organisms without interfering with their hemolytic reactions. Without any additions, the medium can.
  4. Culture and identification of B. mallei was performed as described previously Briefly, nasal swabs, nodule aspirates and venous blood samples collected from the horses were plated onto brain heart infusion agar (BHI; Oxoid Limited, Basingstoke, UK) supplemented with 5% defibrinated sheep blood, BHI-4% glycerol and MacConkey agar (Oxoid Limited.
  5. fresh defibrinated sheep blood, 7% inactivated fetal bovine serum (FBS) and 0.25 mg/ Lof polymyxin B (Sigma), 10 mg/ of vancomycin (Sigma), and 2 mg/L of amphotericin B (Sigma). Then, the cultures were incubated in the anaerobic jar with Gas pack C (Merck) at 37°C for 5 to 7 days. When the bacteria grow, catalase
  6. For expression of flagella, bacteria were grown on trypticase soy agar supplemented with 5% defibrinated sheep blood (Oxoid, Basingstoke, England) at 37°C for 48 h. (Sigma). A 38-kDa protein.

h Interpretative criteria applicable only to tests performed by disk diffusion method using Mueller-Hinton agar with 5% defibrinated sheep blood and incubated in 5% CO2. 3 A report of Susceptible (S) indicates that the antimicrobial drug is likely to inhibit growth of the pathogen if the antimicrobial drug reaches the concentrations. compound amphotericin B (Fungizone; Sigma, catalog no. A2942) was added at a concentration of 10 μg/mL (w/v). 400 μL aliquots of the blood samples were cultured on Columbia Blood Agar Base (CBA) (Difco, catalog no. 279240) supplemented with 5% (v/v) of defibrinated sheep blood. The remaining samples were stored at -80°C for DNA isolation Defibrinated sheep blood was from the Veterinary Research Institute, Gannoruwa, Sri Lanka. UV-1800 UV-Vis spectrophotometer, Shimadzu, Japan and Multiskan Ex plate reader from Thermoscientific, USA, were used for antioxidant and MTT assays respectively

Prior to testing, strains were subcultured three times onto Brucella agar (Becton Dickinson, Le Pont de Claix, France) supplemented with 5% defibrinated sheep blood, 5 mg/L haemin (Sigma, Saint Quentin Fallavier, France) and 1 mg/L vitamin K 1 (Sigma). Antibiotic Bacterial suspensions were mixed with defibrinated sheep blood and assayed for hemolytic activity in 5-min intervals as described for panel A. (C) Quantitation of relative hemolysis over time for the wild-type strain cultured with sulfate (+ sulfate) and without sulfate (− sulfate). Results are representative of three experiments

Rosetteformation byperipheral blood lymphocytesin rheumatoidarthritis form rosettes when mixed with sheep erythrocytes. These spontaneous rosette-forming cells (RFC) he The use of heparinized rather than defibrinated blood increasedtheyield oflymphocytes.Similarly,inclusiono The B henselae strain Marseille 16 was grown on Columbia agar supplemented with 5% defibrinated sheep blood (CBA; Becton Dickinson, Heidelberg, Germany) in a humidified atmosphere at 37°C and 5% CO 2. For production of bacterial stock suspensions, bacteria were harvested after 5 days of culture, resuspended in Luria-Bertani medium containing.

Blood Agar Base No. 2 (Oxoid® CM 271) containing 7% defibrinated sheep blood and Brucella Medium Base (Oxoid®-CM0169) containing Brucella Selective Supplement (Oxoid® SR83) were used for isolation. Abomasum contents and homogenates removed for culture were inoculated on agar plates and incubated in 37 °C and 5% CO 2 conditions for 3-8 days. Cell preparation. Venous blood was obtainedfromhealthy young adults. Lymphocytes were isolated from defibrinated whole blood by dextran sedimentation of the erythrocytes followed by isopyknic centrifugation of the leukocyte-rich supernate on a Ficoll-Hypaque mixture (7). Resultant cell preparations consisted of greater than 90%o lymphocytes Bovine serum albumin agar medium (Merck, Germany) and Tween 80 (Merck, Germany) opacity test medium were used for the assessment of proteinase and esterase activities, respectively. sabouraud dextrose agar supplement with 3% glucose (Merck, Germany) and 7% defibrinated sheep blood was used for the detection of haemolytic activity

S. aureus (NCTC7445, Public Health England, Salisbury, UK) were cultured on tryptone soya agar (SGL, Corby, UK) at 32°C±2°C; S. pyogenes (NCTC12696, Public Health England) were cultured on Columbia blood agar with 5% defibrinated sheep blood (SGL) at 36°C±2°C; M. catarrhalis (NCTC3622, Public Health England) were cultured on Columbia. agar plates containing 5% defibrinated sheep blood, enriched with yeast extract (1 µg/ml), hemin (5 µg/ml), and menadione (10 µg/ml) for 72 h at 37°C. Then, P. gingivalis LPS was prepared by the phenol-water method27 and crude LPS was digested with DNase I and RNase H at 100°C for 15 min, and ultracentrifuged at 100,000 g for 2 h The NIST COVID19-DATA repository is being made available to aid in meeting the White House Call to Action for the Nation's artificial intelligence experts to develop new text and data mining techniques that can help the science community answer high-priority scientific questions related to COVID-19 A volume of 40 μL of cell suspension (initial bacterial count 3×10 8 CFUs/mL) was spread onto the TSA agar plates containing 5% (v/v) defibrinated sheep blood. Final concentrations of ovatodiolide and the various fractions in the medium were set to be 2.0-32.0 μg/mL and 32.0-512.0 μg/mL with DMSO concentration lower than 2.5%, respectively

8806241 1 BBL™ Regan-Lowe Charcoal Agar 8806241 • Rev. 04 • September 2017 QUALITY CONTROL PROCEDURES I INTRODUCTION BD BBL™ Regan-Lowe Charcoal Agar is a selective medium used for the isolation of Bordetella pertussis from clinical specimens. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below The suspension was plated on a 5% defibrinated sheep blood agar plate. The plate was incubated at 37 °C for 18-24 h. Three suspected colonies were injected to separate tryptic soy broth (Sigma Aldrich MFCD00132536) and incubated at 37 °C for 18-24 h. DNA was isolated using 10% chelex-100 (Biorad, CA) [17, 18] P. gingivalis ATCC 33277 was cultured on a blood BHI (brain heart infusion) agar plate containing 40 mg/ml trypot-soya agar (Nissui Pharmaceutical, Tokyo, Japan), 5 mg/ml BHI (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), 1 g/ml cysteine (Wako Pure Chemical Industries, Osaka, Japan), 5μg/ml hemin (Sigma-Aldrich, St. Louis, MO, USA.